Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ca2+ signaling modulates cytolytic T lymphocyte effector functions

M T Esser1, D M Haverstick, C L Fuller

  • 1Department of Microbiology, University of Virginia, Health Sciences Center, Charlottesville, Virginia 22908, USA.

The Journal of Experimental Medicine
|May 16, 1998
PubMed
Summary

Cytolytic T cells use distinct calcium (Ca2+) signaling pathways for perforin/granule exocytosis and Fas ligand/Fas-mediated killing. Differential Ca2+ influx modulates these T cell effector functions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact of Pseudomonas aeruginosa carriage on intensive care unit-acquired pneumonia: a European multicentre prospective cohort study.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2024
Same author

Comparison of immune system development in nonclinical species and humans: Closing information gaps for immunotoxicity testing and human translatability.

Reproductive toxicology (Elmsford, N.Y.)·2019
Same author

DNA purification using dynamic solid-phase extraction on a rotationally-driven polyethylene-terephthalate microdevice.

Analytica chimica acta·2016
Same author

Prostatic Hypertrophy and Treatment by Suprapubic Prostatectomy.

Canadian Medical Association journal·2010
Same author

The impact of a quadrivalent human papillomavirus (types 6, 11, 16, 18) virus-like particle vaccine in European women aged 16 to 24.

Journal of the European Academy of Dermatology and Venereology : JEADV·2009
Same author

High sustained efficacy of a prophylactic quadrivalent human papillomavirus types 6/11/16/18 L1 virus-like particle vaccine through 5 years of follow-up.

British journal of cancer·2006

Area of Science:

  • Immunology
  • Cellular Biology
  • Calcium Signaling

Background:

  • Cytolytic T lymphocytes (CTLs) eliminate target cells through perforin/granule exocytosis or Fas ligand (FasL)/Fas-mediated apoptosis.
  • Both pathways are crucial for immune surveillance and T cell-mediated cytotoxicity.

Purpose of the Study:

  • To investigate the distinct roles of intracellular and extracellular calcium (Ca2+) in TCR-triggered CTL effector functions.
  • To elucidate the differential Ca2+ signaling requirements for perforin/granule exocytosis versus FasL/Fas-mediated killing.

Main Methods:

  • Utilized CD8+ CTL clones with distinct killing mechanisms (perforin/granule and/or FasL/Fas).
  • Employed a panel of Ca2+ antagonists and agonists to manipulate intracellular and extracellular Ca2+ levels.

Related Experiment Videos

  • Measured Ca2+ concentration changes and assessed cytolytic activity.

    • Main Results:

    • Perforin/granule exocytosis requires a large, biphasic increase in intracellular Ca2+, involving both intracellular store release and extracellular influx.
    • FasL/Fas-mediated killing necessitates only a sustained influx of extracellular Ca2+.
    • Low concentrations of Thapsigargin selectively induced FasL/Fas-mediated cytolysis without triggering granule exocytosis.

    Conclusions:

    • Calcium signaling plays a differential role in regulating CTL effector functions.
    • Distinct Ca2+ dynamics dictate the engagement of perforin/granule exocytosis versus FasL/Fas pathways.
    • Understanding these Ca2+ signaling mechanisms can inform strategies to modulate T cell-mediated cytotoxicity.