Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ion binding and chromatin condensation

G A Jacobs, J A Smith, R A Watt

    Biochimica Et Biophysica Acta
    |August 2, 1976
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The enucleated eye test: A comparison of the use of ultrasonic and optic pachometers.

    Toxicology in vitro : an international journal published in association with BIBRA·2010
    Same author

    Citalopram in mentally retarded patients with depression: a long-term clinical investigation.

    European psychiatry : the journal of the Association of European Psychiatrists·2001
    Same author

    Extraction of sensory parameters from a neural map by primary sensory interneurons.

    The Journal of neuroscience : the official journal of the Society for Neuroscience·2001
    Same author

    MSNJ Alliance: looking back on the past year.

    New Jersey medicine : the journal of the Medical Society of New Jersey·2000
    Same author

    Neural mapping of direction and frequency in the cricket cercal sensory system.

    The Journal of neuroscience : the official journal of the Society for Neuroscience·1999
    Same author

    Neural mechanisms of behavioral plasticity: metamorphosis and learning in Manduca sexta.

    Brain, behavior and evolution·1997
    Same journal

    Cumulative Contents.

    Biochimica et biophysica acta·2020
    Same journal

    Molecular Basis of Disease Cumulative Contents.

    Biochimica et biophysica acta·2020
    Same journal

    General Subjects Cumulative Contents.

    Biochimica et biophysica acta·2020
    Same journal

    Erratum to 'on the role of exchangeable hydrogen bonds for the kinetics of P680<sup>+·</sup> Q<sub>A</sub> <sup>-·</sup> formation and P680<sup>+·</sup> Pheo<sup>-·</sup> recombination in photosystem II' [Biochim. Biophys. Acta 1276 (1996) 35-44].

    Biochimica et biophysica acta·2019
    Same journal

    Oligomeric state of the light-harvesting complexes B800-850 and B875 from purple bacterium Rubrivivax gelatinosus in detergent solution.

    Biochimica et biophysica acta·2019
    Same journal

    Regulation of pigment content and enzyme activity in the cyanobacterium Nostoc sp. Mac grown in continuous light, a light-dark photoperiod, or darkness.

    Biochimica et biophysica acta·2019
    See all related articles

    Calcium ions (Ca2+) binding to hen erythrocyte chromatin causes reversible condensation. This chromatin condensation is linked to calcium binding, suggesting cation neutralization of DNA phosphate repulsion maintains chromatin structure.

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Cell Biology

    Background:

    • Chromatin structure and condensation are crucial for cellular processes.
    • Cation binding plays a significant role in modulating chromatin organization.
    • Understanding calcium's role in chromatin condensation provides insights into cellular regulation.

    Purpose of the Study:

    • To investigate the binding of 45Ca2+ to hen erythrocyte chromatin.
    • To elucidate the mechanism by which cations induce reversible chromatin condensation.
    • To explore the relationship between calcium binding and chromatin condensation states.

    Main Methods:

    • Studied the binding of radioactive calcium-45 (45Ca2+) to isolated hen erythrocyte chromatin.
    • Quantified calcium binding using stability constants and saturation values.

    Related Experiment Videos

  • Correlated calcium binding levels with the degree of chromatin condensation.
  • Investigated cation competition for calcium binding sites.

    • Main Results:

    • Calcium (Ca2+) binds to hen erythrocyte chromatin with a stability constant of approximately 3.1.
    • The saturation value for Ca2+ binding is 0.25 Ca2+ per DNA phosphate, half that of pure DNA.
    • Chromatin condensation is half complete when calcium binding is approximately half complete.
    • The transition to condensed chromatin occurs as bound cations neutralize repulsion between DNA phosphates.

    Conclusions:

    • Cation binding, specifically calcium, is a key factor in the reversible condensation of erythrocyte chromatin.
    • The observed calcium binding stoichiometry supports models of chromatin structure involving DNA wrapped around histones.
    • Genetically active chromatin may remain uncondensed in vivo due to unneutralized negative groups at physiological cation concentrations.