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Related Experiment Videos

Postganglionic sympathetic neurons express endothelin

D H Damon1

  • 1Department of Pharmacology, University of Vermont, Burlington 05405, USA.

The American Journal of Physiology
|April 8, 1998
PubMed
Summary

Endothelin (ET) is a peptide found in sympathetic neurons. These neurons synthesize and secrete ET-1 and ET-3, suggesting a role in neurotransmission.

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Endocrinology

Background:

  • Endothelin (ET) is a peptide initially identified for its vasoconstrictive properties.
  • ET is now known to be present and active in various tissues, including the central nervous system, where it may influence neurotransmission.

Purpose of the Study:

  • To investigate the synthesis and secretion of Endothelin (ET) by postganglionic sympathetic neurons.
  • To determine if sympathetic neurons produce ET-1 and ET-3.

Main Methods:

  • Northern analysis to detect ET-1 mRNA in cultured superior cervical ganglion (SCG) neurons.
  • Immunohistochemistry to identify ET-1 and ET-3 peptides in SCG neurons.
  • Radioimmunoassay (RIA) to quantify ET-1 and ET-3 levels in cultured SCG media and adult SCG extracts.

Main Results:

  • ET-1 mRNA was detected in cultured SCG neurons.
  • ET-1 and ET-3 peptides were identified in cultured SCG neurons.
  • Quantifiable amounts of ET-1 and ET-3 were measured in both conditioned media from cultured SCG neurons and extracts from adult SCG.

Conclusions:

  • Postganglionic sympathetic neurons synthesize and secrete Endothelin peptides.
  • The findings suggest a potential role for Endothelin in sympathetic neurotransmission.

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