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Cloning and sequencing of complementary DNA for fatty acid binding protein from rainbow trout heart

S Ando1, X H Xue, G F Tibbits

  • 1Department of Biological Sciences, Simon Fraser University, Burnaby, B.C., Canada.

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
|April 8, 1998
PubMed

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Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
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