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Characterization of Cyprinus carpio brain nitric oxide synthase
1Department of Animal Biology, University of Modena, Italy.
Neuroscience Letters
|April 8, 1998
Abstract:
Nitric oxide synthase (NOS) activity is found both in soluble and in particulate fractions of the carp brain. The Km values for arginine are 2.8+/-0.5 and 3.3+/-0.4 microM for the soluble and particulate fractions, respectively. K for NG-monomethyl-L-arginine inhibitor are 2.6+/-0.5 and 2.9+/-0.6 microM, and activation energy for the breakdown of the substrate-enzyme complex 8120+/-710 and 4620+/-450 cal per mole. Carp enzyme shows higher affinity than rat NOS for Ca2+ and for the competitive inhibitor 7-nitroindazole.