Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Phenotype-dependent differences in proteasome subunit composition and cleavage specificity in B cell lines

T Frisan1, V Levitsky, A Polack

  • 1Microbiology and Tumor Biology Center, Karolinska Institute, Stockholm, Sweden. teresa.frisan@mtc.ki.se

Journal of Immunology (Baltimore, Md. : 1950)
|April 8, 1998
PubMed
Summary

Burkitt

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bacterial genotoxin functions as immune-modulator and promotes host survival.

Microbial cell (Graz, Austria)·2017
Same author

Oxidative stress enables Epstein-Barr virus-induced B-cell transformation by posttranscriptional regulation of viral and cellular growth-promoting factors.

Oncogene·2015
Same author

Telomere dysfunction and activation of alternative lengthening of telomeres in B-lymphocytes infected by Epstein-Barr virus.

Oncogene·2013
Same author

The Epstein-Barr virus nuclear antigen-1 promotes telomere dysfunction via induction of oxidative stress.

Leukemia·2011
Same author

The photochemical substance of the renal cones and the theory of colored sensations.

Comptes rendus hebdomadaires des seances de l'Academie des sciences·2010
Same author

Three Epstein-Barr virus latency proteins independently promote genomic instability by inducing DNA damage, inhibiting DNA repair and inactivating cell cycle checkpoints.

Oncogene·2009

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • The 26S proteasome is a crucial cellular machine for protein degradation, influencing various cellular processes.
  • B cell lymphomas, such as Burkitt's lymphoma (BL), exhibit distinct cellular phenotypes compared to normal B cells.
  • Interferon-gamma (IFN-gamma) regulates proteasome subunit composition and activity, impacting antigen presentation.

Purpose of the Study:

  • To compare the subunit composition and enzymatic activity of 26S proteasomes in Burkitt's lymphoma (BL) cells versus normal B cell lines (EBV-transformed lymphoblastoid cell lines, LCLs).
  • To investigate the role of specific proteasome subunits (Lmp2, Lmp7, MECL-1) in BL cells and their correlation with proteasome activity.
  • To assess the impact of cellular differentiation and transformation on proteasome function and peptide cleavage.

Related Experiment Videos

Main Methods:

  • Purification of 26S proteasomes from BL and LCL cell lines.
  • Immunoprecipitation and two-dimensional gel electrophoresis to analyze proteasome subunit composition.
  • Enzymatic activity assays using fluorogenic substrates and a specific peptide analogue (E4416-424Y).

Main Results:

  • BL cells showed low expression of IFN-gamma-regulated proteasome subunits (Lmp2, Lmp7, MECL-1) compared to LCLs.
  • Proteasomes from BL cells exhibited reduced chymotrypsin- and trypsin-like activities.
  • BL proteasomes efficiently cleaved a specific EBV nuclear antigen peptide (E4416-424Y), while LCL proteasomes did not.

Conclusions:

  • B cell lines at different differentiation stages possess proteasomes with distinct subunit compositions and enzymatic activities.
  • These proteasome variations in BL cells may lead to the generation of unique endogenous peptides.
  • Altered proteasome function in BL cells could influence their immunogenicity and T-cell recognition.