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Epitope-specific antibody response to IgE by mimotope immunization
M P Rudolf1, M Vogel, F Kricek
1Institute of Immunology and Allergology, University of Bern, Inselspital, Switzerland.
Journal of Immunology (Baltimore, Md. : 1950)
|April 8, 1998
Summary
Researchers identified specific peptide mimotopes that bind to the anti-human IgE antibody BSW17. These mimotopes show potential for developing new immunotherapies by inducing a beneficial anti-IgE response.
Area of Science:
- Immunology
- Allergy Research
- Therapeutic Antibody Development
Background:
- A mouse monoclonal antibody, BSW17, recognizes receptor-bound human IgE without triggering mediator release.
- BSW17-IgE immune complexes do not bind to the IgE receptor.
Purpose of the Study:
- To precisely map the epitope recognized by the BSW17 antibody.
- To identify novel peptide mimotopes that mimic the BSW17 epitope.
- To explore the therapeutic potential of these mimotopes for anti-IgE immunotherapy.
Main Methods:
- Screening of random peptide phage display libraries to isolate mimotopes.
- Characterization of mimotope binding specificity using various anti-IgE antibodies.
- Assessment of mimotope-IgE interactions and carrier dependency.
- Immunization of rabbits with phage-displayed mimotopes to generate specific anti-IgE responses.
Main Results:
- Isolation of circular nona- and octapeptides (mimotopes) specifically recognized by BSW17.
- These mimotopes inhibited human IgE binding to BSW17 but not to other anti-IgE antibodies.
- Carrier-independent binding of cyclic peptides was confirmed.
- Rabbit immunization with mimotopes induced an anti-IgE response specific to the BSW17 epitope.
Conclusions:
- Constrained peptides (mimotopes) can effectively mimic conformational epitopes.
- These mimotopes are highly specific for the BSW17 antibody's target epitope.
- Mimotopes or derived peptides hold promise for developing novel immunotherapies to induce beneficial in vivo anti-IgE responses.