Long-term intracranial cannula stabilization in mice with light cured resin composites

A simple and rapid method is described for the introduction and stabilization of chronic indwelling cannulae in mice. Materials and adhesive bonding techniques commonly used in restorative dentistry were used to stabilize the cannulae. The effectiveness of this method was evaluated by shear strength testing across time and histological evaluation of coronal brain sections. Shear strength increased during the first week post surgery and remained stable for up to 60 days. The force required to dislodge the cannulae from skulls ranged from 0.70 to 2.45 kg. Histological evaluation revealed no significant infection or inflammatory response due to the materials used. Cannulae stabilization by this technique appears to be suitable for most experimental conditions.