[Polymorphisms of four microsatellite markers closely linked with PKD1 in Chinese]

To investigate the polymorphisms and evaluate the value in gene diagnosis of four microsatellites, which are the KG8 that locating in 3'untranslated region of PKD1 and SM6, CW4 and CW2, closely linked to PKD1.

The DNAs of some unrelated Chinese Han people were analyzed by using PCR, PAGE and silver staining and the findings were compared with those reported on Caucasian.

In Chinese, KG8 included 6 alleles and PIC was 0.312; in Caucasian,8 alleles,PIC 0.545. The number of alleles and PIC of KG8 in Chinese were not so many as those in Caucasian. SM6 included 24 kinds of alleles and PIC was 0.80 in Chinese; in Caucasian,16 alleles, PIC 0. 653. CW4 is 9 alleles in Chinese and PIC of SM6 were more than those in Caucasian. In Chinese CW4, 9 alleles, PIC 0.850; in Caucasian, 9 alleles, PIC 0.782. Although the number of CW4 allele in the two nations was the same, PIC in Chinese was higher than that in Caucasian. In Chinese CW2,7 alleles, PIC 0.814; and in Caucasian, 13 alleles, PIC 0.809. The number of allele of CW2 in Chinese was not so many as that in Caucasian; however,the PIC was about the same.

The results indicate that there are differences in the number of allele and distribution of the four microsatellite markers between Chinese and Caucasian. So there exists difference of dinucleotide repeat between two nations. In addition,SM6, CW4 and CW2 could be used as valuable markers in gene linkage diagnosis and personal and related identification in forensic medicine.