Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Different heparin fractions]

S M Bychkov, V N Kharlamova

    Biulleten' Eksperimental'Noi Biologii I Meditsiny
    |March 1, 1976
    PubMed
    Summary

    Treatment with hexamminecobalt(III) separated unfractionated heparin into two distinct fractions based on their unique sulfation patterns. This chemical separation method yields purified heparin with varying degrees of sulfation for potential applications.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Proteoglycans and cells].

    Biulleten' eksperimental'noi biologii i meditsiny·1996
    Same author

    [Function of carboxyl and sulfate groups in proteoglycans].

    Biulleten' eksperimental'noi biologii i meditsiny·1995
    Same author

    [The role of protein components of proteoglycans in steric exclusion of cells].

    Biulleten' eksperimental'noi biologii i meditsiny·1994
    Same author

    [Erythrocyte aggregation in the blood in different states of the animal organism].

    Biulleten' eksperimental'noi biologii i meditsiny·1993
    Same author

    [Action of proteoglycans on erythrocytes in circulating blood].

    Biulleten' eksperimental'noi biologii i meditsiny·1993
    Same author

    [The mechanism of the steric exclusion of cells brought about by proteoglycans].

    Biulleten' eksperimental'noi biologii i meditsiny·1992

    Area of Science:

    • Biochemistry
    • Chemical Separation
    • Glycosaminoglycan Chemistry

    Context:

    • Heparin is a complex mixture of glycosaminoglycans with significant biological activity.
    • Characterizing and separating heparin fractions is crucial for understanding its structure-function relationships.
    • Existing methods for heparin fractionation can be complex and may alter its native structure.

    Purpose:

    • To investigate the efficacy of hexamminecobalt(III) in fractionating unfractionated heparin preparations.
    • To characterize the chemical composition of separated heparin fractions, specifically their sulfation levels.
    • To isolate chemically individual heparin fractions with distinct structural properties.

    Summary:

    • Treatment of unfractionated heparin with 2-3 M KCl solutions containing hexamminecobalt(III) selectively precipitated one heparin fraction.
    • The soluble heparin fraction exhibited a sulfation ratio of 3:1 (sulfuric acid residues to glucosamine residues).
    • The precipitated heparin fraction showed a higher sulfation ratio of 4:1 (sulfuric acid residues to glucosamine residues), and both fractions were isolated in a chemically pure form.

    Impact:

    • This study presents a novel chemical method for heparin fractionation based on differential sulfation.
    • The isolation of distinct heparin fractions with defined sulfation patterns can facilitate targeted therapeutic development.
    • Provides a foundation for further research into the biological activities of specifically sulfated heparin molecules.

    Related Experiment Videos