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Recommended storage and resuscitation conditions for the MDCC-MSB1 cell line
1Biomedical Research Group, School of Biomedical Sciences, Charles Sturt University, Wagga Wagga, New South Wales, Australia.
Avian Diseases
|April 9, 1998
Abstract:
Storage and resuscitation of MDCC-MSB1 cells, most commonly used for the propagation of chicken anemia virus was studied. Cells were frozen slowly in RPMI medium 1640 with 10% or 20% fetal bovine serum and 20% dimethyl sulfoxide, held at -70 C overnight, and placed into liquid nitrogen the next day. After 1 mo, 91% to 100% resuscitation rates were obtained, while cells that were frozen fast (directly transferred into liquid nitrogen) could not be resuscitated.