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Occurrence of 13(S)-hydroxyoctadecadienoic acid in biological samples
S A Spindler1, K S Clark, M L Blackburn
1Oxford Biomedical Research, Inc, Rochester Hills, MI 48309-3611, USA.
Abstract:
The oxygenated metabolite of linoleic acid, 13(S)-hydroxyoctadecadienoic acid has recently been shown to play a role in cellular regulation. To detect this molecule in biological systems, we recently developed a specific polyclonal antibody. Using this antibody, we report the presence of 13(S)-hydroxyoctadecadienoic acid in human urine, cell culture media, and untreated goat serum for the first time by a specific, sensitive, and rapid enzyme immunoassay. Furthermore, the enzyme linked immunosorbent assay data are verified by gas chromatography/mass spectrometry analysis of the same samples.
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