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Heparan sulfate proteoglycans recognize ghost Pick bodies

T Odawara1, E Iseki, F Li

  • 1Department of Psychiatry, Yokohama City University School of Medicine, Yokohama, Japan.

Neuroscience Letters
|April 9, 1998
PubMed
Summary

The monoclonal antibody 3G10 identifies ghost Pick bodies (PBs) better than intracellular PBs in Pick's disease. Heparan sulfate proteoglycans are implicated in the extinction process of these cellular structures.

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Area of Science:

  • Neuropathology
  • Neurodegenerative Diseases
  • Cell Biology

Background:

  • Pick's disease is characterized by the accumulation of tau-positive Pick bodies (PBs).
  • The precise nature and formation process of PBs, particularly their potential degradation, remain incompletely understood.

Purpose of the Study:

  • To investigate the role of heparan sulfate proteoglycans (HSPGs) in the formation and potential clearance of Pick bodies.
  • To characterize novel structures identified by an anti-HSPG antibody in the context of Pick's disease pathology.

Main Methods:

  • Immunohistochemistry using a monoclonal antibody (3G10) against HSPGs and an anti-tau antibody.
  • Immunoelectron microscopy to examine the ultrastructure of identified deposits.
  • Analysis of affected brain regions including the dentate gyrus and temporal cortex.

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Main Results:

  • The anti-HSPG antibody 3G10 recognized irregular round structures (IRSs) adjacent to tau-positive intracellular PBs.
  • Some IRSs were also weakly positive for tau, and only partially co-localized with intracellular PBs.
  • Electron microscopy revealed IRSs as aggregated fibrillary structures (10-20 nm diameter), consistent with 'ghost' PBs.
  • 3G10 appears to identify ghost PBs more effectively than intracellular PBs.

Conclusions:

  • Heparan sulfate proteoglycans are involved in the formation or persistence of ghost PBs.
  • The findings suggest a role for HSPGs in the process of Pick body extinction or modification.
  • The antibody 3G10 serves as a valuable tool for studying PB pathology and their potential clearance mechanisms.