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Related Experiment Videos

Chronic morphine alters calbindin D-28k immunostaining patterns in mouse forebrain

P Maharajan1, R Prencipe, R Falchetti

  • 1CNR Institute of Cybernetics, Naples, Italy.

Neuroscience Letters
|April 16, 1998
PubMed
Summary

Chronic morphine exposure in female mice reduced calbindin D-28k cells in specific brain regions. This neurochemical change may impact central nervous system function in adults.

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Area of Science:

  • Neuroscience
  • Pharmacology
  • Toxicology

Background:

  • Chronic morphine use can lead to neuroadaptations.
  • Calbindin D-28k is a neuroprotective calcium-binding protein found in specific brain regions.

Purpose of the Study:

  • To investigate the effects of chronic morphine exposure on adult mouse brain histology and calbindin D-28k expression.
  • To determine if morphine treatment during sensitive developmental and adult periods alters neurochemical markers.

Main Methods:

  • Female Swiss mice received daily saline or morphine (30 or 60 mg/kg) for 7 days pre-mating, during gestation, and until 21 days post-partum.
  • Brains were perfusion-fixed and analyzed using histology and calbindin D-28k protein-immunoreactivity.

Main Results:

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  • No significant histological changes were observed in various brain regions.
  • A reduction in calbindin-positive cells was found in the cingulate cortex, parietal cortex, and lateral septal regions of morphine-treated mice compared to controls.

Conclusions:

  • Chronic morphine administration alters calbindin D-28k expression in specific brain areas of adult mice.
  • Changes in this neuroprotective protein may contribute to the central nervous system modifications induced by addictive drugs.