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Analysis of colchicine-induced chromosomal aberrations in embryos obtained from XO mice
1Department of Hygiene and Preventive Medicine, Yamagata University School of Medicine, Japan. hisikawa@med.id.yamagata-u.ac.jp
Abstract:
A high incidence of chromosomal anomalies has been observed in the offspring of our XO mouse colony. However, the reason(s) are unknown. We hypothesized that XO dams might be more susceptible to certain chromosomal mutagens. Therefore, we assessed whether the oocytes of XO mice are more susceptible to colchicine. Pregnant XO and XX mice were intraperitoneally (i.p.) injected with colchicine, 0.2 mg/kg, 6 h after mating (between resumption of meiosis II and extrusion of the second polar body). Contrary to our expectation, the incidence of chromosomal anomalies induced by colchicine exposure did not differ between XX and XO dams. These findings suggest that the chromosomal stability of the oocytes from XO mice may not be affected by colchicine exposure at the stage of extrusion of the second polar body. The effects of chromosomal imbalance inherent in XO mice on chemical susceptibilities should be further investigated.
Insights
XO mice dams did not show increased chromosomal anomalies when exposed to colchicine, contrary to expectations. Further research is needed to understand XO mice
Area of Science:
- Reproductive biology
- Genetics
- Toxicology
Background:
- XO mice exhibit a high incidence of chromosomal anomalies in offspring.
- The underlying causes for these anomalies remain unknown.
- A hypothesis suggests XO dams may be more susceptible to chromosomal mutagens.
Purpose of the Study:
- To investigate if oocytes from XO mice are more susceptible to the mutagen colchicine.
- To assess the impact of colchicine on chromosomal stability in XO mouse oocytes.
Main Methods:
- Pregnant XO and XX mice were administered intraperitoneal injections of colchicine (0.2 mg/kg).
- Injections were given 6 hours post-mating, during meiosis II resumption and second polar body extrusion.
- Chromosomal anomalies were analyzed in oocytes from both XX and XO dams.
Main Results:
- Colchicine exposure did not result in a differing incidence of chromosomal anomalies between XX and XO dams.
- These findings indicate colchicine does not uniquely affect chromosomal stability in XO oocytes at this specific meiotic stage.
Conclusions:
- XO mouse oocyte chromosomal stability is not demonstrably affected by colchicine at the stage of second polar body extrusion.
- Further investigation is required to explore the impact of inherent chromosomal imbalance in XO mice on susceptibility to chemical agents.