Related Experiment Video

Updated: Aug 11, 2026

Analysis of RNA Processing Reactions Using Cell Free Systems: 3' End Cleavage of Pre-mRNA Substrates in vitro
09:16

Analysis of RNA Processing Reactions Using Cell Free Systems: 3' End Cleavage of Pre-mRNA Substrates in vitro

Published on: May 3, 2014

Coupling termination of transcription to messenger RNA maturation in yeast

C E Birse1, L Minvielle-Sebastia, B A Lee

  • 1Sir William Dunn School of Pathology, South Parks Road, University of Oxford, Oxford OX1 3RE, UK.

Science (New York, N.Y.)
|May 2, 1998
PubMed

Insights

Messenger RNA (mRNA) 3'-end processing and transcription termination are directly linked. Cleavage factors, not polyadenylation factors, are crucial for coupling these processes for the CYC1 gene.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Messenger RNA (mRNA) processing is essential for gene expression.
  • Transcription termination is a critical step in gene regulation.
  • The interplay between mRNA 3 -end processing and transcription termination is not fully understood.

Purpose of the Study:

  • To investigate the direct association between mRNA 3 -end processing and transcription termination.
  • To determine the roles of specific processing factors in this linkage.

Main Methods:

  • Utilized the CYC1 gene in Saccharomyces cerevisiae.
  • Introduced mutations in genes encoding factors for mRNA cleavage and polyadenylation.
  • Assessed the impact of these mutations on transcription termination.

Main Results:

  • Mutations in cleavage factors (RNA14, RNA15, PCF11) disrupted transcription termination.
  • Mutations in polyadenylation factors (PAP1, FIP1, YTH1) had minimal effect on termination.
  • Established a direct link between cleavage factors and transcription termination.

Conclusions:

  • Cleavage factors play a critical role in coupling transcription termination with pre-mRNA 3 -end processing.
  • This linkage is mediated by factors involved in the initial cleavage step.
  • RNA polymerase II transcription termination is directly influenced by mRNA 3 -end cleavage machinery.

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