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Human lymphoblastoid cell line producing specific antibody against Rh-antigen D
1Finnish Red Cross Blood Transfusion Service, University of Helsinki, Finland.
Scandinavian Journal of Immunology
|January 1, 1980
Summary
Researchers established a cell line producing specific anti-Rh D antibody using Epstein-Barr virus (EBV) infection. This method enriches antibody-producing cells, increasing antibody levels for potential therapeutic applications.
Area of Science:
- Immunology
- Cell Biology
- Virology
Background:
- Development of specific antibody-producing cell lines is crucial for research and therapeutics.
- RhD antibodies are important in transfusion medicine and hemolytic disease of the newborn.
Observation:
- Lymphocytes from an immunized donor with high anti-D antibody levels were used.
- Cells were preselected using Rh-positive erythrocytes before Epstein-Barr virus (EBV) infection.
- The resulting lymphoblastoid cell line produced specific IgG1 anti-D antibody.
Findings:
- The established cell line demonstrated specific antibody production against the RhD determinant.
- Nonspecific IgM and IgG antibodies were also produced by the cell line.
- Rerosetting the cells enriched the specific antibody-producing population and increased antibody titer in the supernatant.
Implications:
- This EBV-transformed cell line provides a valuable tool for studying anti-RhD antibody production.
- Enrichment strategies can enhance the yield of specific antibodies for potential clinical use.
- The methodology may be applicable to generating other specific antibody-producing cell lines.