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Mouse/human T-cell hybrids rosetting with sheep erythrocytes
H A Suomalainen1, R A Goldsby, B A Osborne
1Folkhälsan Institute of Genetics, Helsinki, Finland.
Scandinavian Journal of Immunology
|January 1, 1980
Summary
Human chromosome 6 is linked to sheep red blood cell (SRBC) rosetting in hybrid cells. Genes on chromosome 6 appear responsible for this cell-surface interaction.
Area of Science:
- Immunology
- Cell Biology
- Genetics
Background:
- Hybrid cells are created by fusing different cell types.
- Understanding gene function requires studying chromosome contributions.
Purpose of the Study:
- To investigate the genetic basis of sheep red blood cell (SRBC) rosetting in hybrid cells.
- To identify specific human chromosomes involved in SRBC binding.
Main Methods:
- Fusing concanavalin-A-activated human lymphocytes with AKR mouse thymoma cells (BW 5147).
- Culturing hybrid cells and analyzing their chromosome content over six months.
- Assessing SRBC rosetting levels in hybrid cell clones with varying human chromosome complements.
Main Results:
- Twenty-seven of forty-nine hybrid cell clones retained human chromosomes after six months.
- Human chromosome 6 was present in 89% of clones, and human X in 70%.
- Clones with human chromosome 6 showed significantly higher SRBC rosetting, with some clones exhibiting this trait solely due to chromosome 6.
Conclusions:
- Genes located on human chromosome 6 are strongly implicated in mediating sheep red blood cell (SRBC) rosetting.
- Human chromosome 6 plays a crucial role in the observed cell-surface interactions in these hybrid cells.