Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Thymic lymphomas in mice with a truncating mutation in Brca2

L S Friedman1, F C Thistlethwaite, K J Patel

  • 1Cancer Research Campaign, Human Cancer Genetics Research Group, Cambridge University, Addenbrooke's Hospital, United Kingdom.

Cancer Research
|April 16, 1998
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Social determinants of health inequalities in early phase clinical trials in Northern England.

British journal of cancer·2024
Same author

EPIC: an evaluation of the psychological impact of early-phase clinical trials in cancer patients.

ESMO open·2022
Same author

Determinants of SARS-CoV-2 receptor gene expression in upper and lower airways.

medRxiv : the preprint server for health sciences·2020
Same author

Science in Focus: Genomic Instability and its Implications for Clinical Cancer Care.

Clinical oncology (Royal College of Radiologists (Great Britain))·2018
Same author

Cyclin G1 regulates the outcome of taxane-induced mitotic checkpoint arrest.

Oncogene·2011
Same author

A mitotic function for the high-mobility group protein HMG20b regulated by its interaction with the BRC repeats of the BRCA2 tumor suppressor.

Oncogene·2011

This study created a Brca2 gene mutation in mice, leading to early death in most homozygous mutants. Surviving adults developed thymic lymphomas, highlighting Brca2

Area of Science:

  • Genetics
  • Cancer Biology
  • Mouse Models

Background:

  • Mutations in the BRCA2 gene are linked to hereditary breast and ovarian cancers in women.
  • Understanding Brca2 function in vivo is crucial for cancer research.

Purpose of the Study:

  • To investigate the in vivo function of the Brca2 gene.
  • To establish a mouse model for Brca2-associated cancers.

Main Methods:

  • Generated a targeted mutation in the mouse Brca2 gene (Brca2(tm1Cam)) by introducing a premature stop codon in exon 11.
  • Phenotypic analysis of homozygous and heterozygous Brca2 mutant mice.

Main Results:

  • Brca2(tm1Cam) homozygous mutant mice exhibit high lethality, with 90% dying prenatally or perinatally.

Related Experiment Videos

  • Adult homozygous mutants that survived developed thymic lymphomas between 12-14 weeks of age.
  • Heterozygous mice showed no tumor development up to 10 months.

    • Conclusions:

    • The Brca2(tm1Cam) mutation causes early lethality and thymic lymphomas in mice, mirroring human BRC A2-associated cancer phenotypes.
    • This mouse model is valuable for studying Brca2 function and developing cancer therapies.