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Related Experiment Videos

High-precision continuous-flow isotope ratio mass spectrometry

J T Brenna1, T N Corso, H J Tobias

  • 1Division of Nutritional Sciences, Cornell University, Ithaca, New York 14853, USA. JTB4@CORNELL.EDU

Mass Spectrometry Reviews
|April 16, 1998
PubMed
Summary

Continuous-flow techniques have revolutionized isotope ratio mass spectrometry (IRMS), enabling high-precision measurements of C, N, O, S, and H isotopes. Advances include automated analysis of various sample types and compound-specific isotope analysis (CSIA).

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Area of Science:

  • Analytical Chemistry
  • Environmental Science
  • Geochemistry

Background:

  • High-precision isotope determinations are crucial across natural sciences.
  • Instrument principles for isotope ratio mass spectrometry (IRMS) have seen limited change for 40 years.

Purpose of the Study:

  • To highlight the impact of continuous-flow techniques on IRMS capabilities.
  • To showcase advancements in high-precision isotopic analysis for various elements and sample types.
  • To introduce novel methods for compound-specific and position-specific isotope analysis.

Main Methods:

  • Interfacing elemental analyzers with IRMS for C and N isotopic analysis.
  • Utilizing gas/liquid equilibrators for automated O and H isotopic analysis of aqueous fluids.
  • Employing automated cryogenic concentrators for trace-level environmental sample analysis.

Related Experiment Videos

  • Integrating capillary gas chromatography with IRMS for compound-specific isotope analysis (CSIA).
  • Demonstrating GC- and LC-based interfaces for CSIA of H and other analytes.
  • Applying automated position-specific isotope analysis (PSIA) using noncatalytic pyrolysis.

    • Main Results:

    • Continuous-flow techniques have significantly expanded IRMS capabilities since the 1990s.
    • Routine analysis of unprocessed samples for C and N isotopes is now feasible.
    • Automated analysis of O and H isotopes in complex aqueous samples is established.
    • Trace-level analysis in environmental samples is enabled by cryogenic concentrators.
    • CSIA for C, N, and O at low analyte concentrations (1 nmol) has been achieved.
    • PSIA shows promise for intramolecular isotope ratio analysis without significant scrambling.

    Conclusions:

    • Continuous-flow IRMS has transformed the field, offering unprecedented precision and versatility.
    • Automated and specialized techniques like CSIA and PSIA are advancing isotopic analysis.
    • Ongoing developments promise wider routine application of these sophisticated methods.