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Updated: Aug 17, 2026
Detecting the Lyme Disease Spirochete, Borrelia Burgdorferi, in Ticks Using Nested PCR
Published on: February 4, 2018
Ehrlichial DNA amplified from Ixodes ricinus (Acari: Ixodidae) in France
1Unité des Rickettsies, WHO Collaborative Center for Rickettsial Reference and Research, CNRS UPRES A 6020, Faculté de Médećine, Marseille, France.
Abstract:
Granulocytic ehrlichia 16S rDNA was amplified for the 1st time from an Ixodes ricinus (Linne) tick collected in Europe. Sequence analysis of polymerase chain reaction products from the 16S rRNA gene demonstrated the organism from which it originated to be closely related to the agent of human granulocytic ehrlichiosis, an emerging disease that was recently described in the United States; Ehrlichia phagocytophila, the agent of tick-borne fever of ruminants in Europe; and Ehrlichia equi. the agent of the worldwide equine granulocytic ehrlichiosis. These granulocytic ehrlichiae have been associated with Ixodes spp. ticks that may act as vectors. It remains to be determined if each of these granulocytic ehrlichiae, that may constitute variants of the same species, is responsible for a specific disease in animals and in humans.
