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Oocyte transfer in mares

K Hinrichs1, G L Matthews, D A Freeman

  • 1Section of Large Animal Clinical Sciences, School of Veterinary Medicine, Tufts University, North Grafton, MA 01536, USA.

Journal of the American Veterinary Medical Association
|April 16, 1998
PubMed
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Oocyte transfer in mares following in vitro maturation shows promising results for equine reproduction. This study indicates potential clinical applications for equine assisted reproductive technologies.

Area of Science:

  • Veterinary Science
  • Reproductive Biology
  • Equine Medicine

Background:

  • Successful equine reproduction relies on efficient oocyte maturation and fertilization.
  • Assisted reproductive technologies (ART) are crucial for managing equine breeding programs and genetic preservation.

Purpose of the Study:

  • To evaluate the efficacy of in vitro matured oocyte transfer in achieving pregnancies in recipient mares.
  • To determine optimal in vitro maturation times for equine oocytes.

Main Methods:

  • Oocytes were collected from donor mares 24 hours post-human chorionic gonadotropin (hCG) administration.
  • In vitro oocyte maturation was performed for 12 or 18 hours.
  • Oocytes were transferred to recipient mares, with maturation times post-hCG of 36 and 42 hours.

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Main Results:

  • Pregnancy rates were 4/5 (80%) for the 36-hour maturation group and 2/3 (66.7%) for the 42-hour group.
  • An overall pregnancy rate of 6/8 (75%) mares was achieved.
  • These results demonstrate successful pregnancies following the transfer of in vitro matured oocytes.

Conclusions:

  • Equine oocyte transfer after in vitro maturation is a viable technique for establishing pregnancy.
  • The study suggests that oocyte transfer may offer a valuable clinical application in equine reproduction.
  • Further research can optimize maturation protocols to enhance pregnancy rates in mares.