Kinetic urine creatinine determination with the Gemsaec analyzer

Area of Science:

• Clinical Chemistry
• Analytical Chemistry
• Biochemistry

Background:

• Urinary creatinine measurement is crucial for assessing kidney function.
• Traditional methods can be time-consuming and prone to interference.
• Need for rapid, automated, and reliable analytical techniques.

Purpose of the Study:

• To describe a novel, rapid, automated method for urinary creatinine determination.
• To evaluate the method's ability to circumvent common interfering substances.
• To compare the performance of the automated method with existing techniques.

Main Methods:

• Utilized the Jaffé reaction for creatinine quantification.
• Employed reaction rate kinetics to mitigate interferences from protein, glucose, and ketones.
• Implemented a centrifugal analyzer (ENI-Gemsaec) for automated analysis.

Main Results:

• The automated method demonstrated effective circumvention of protein, glucose, and ketone interferences.
• Achieved precision and accuracy comparable to manual Jaffé methods with deproteinization.
• Performance was also comparable to the standard Auto-Analyzer technique.

Conclusions:

• The described automated centrifugal method provides a rapid and reliable approach for urinary creatinine analysis.
• Reaction rate kinetics are key to overcoming interferences in automated Jaffé assays.
• This method offers a viable alternative to traditional techniques for clinical laboratories.