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Related Experiment Videos

Modified nucleosides in urine: selective removal and analysis

M Uziel, L H Smith, S A Taylor

    Clinical Chemistry
    |September 1, 1976
    PubMed
    Summary

    A novel phenylboronic acid adsorbent gel enables rapid, simple quantitation of urinary nucleosides. This method enhances cancer detection by specifically measuring cancer-associated nucleosides like pseudouridine.

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    Area of Science:

    • Biochemistry
    • Analytical Chemistry
    • Oncology

    Background:

    • Urinary nucleoside excretion increases in all cancer types.
    • Accurate quantitation of these nucleosides is crucial for cancer detection and treatment.
    • Existing methods for nucleoside analysis can be complex and time-consuming.

    Purpose of the Study:

    • To develop a rapid and simple method for quantitating urinary nucleosides.
    • To create a specific adsorbent for nucleoside analysis in urine.
    • To utilize this method for improved cancer diagnostics.

    Main Methods:

    • Preparation of an affinity adsorbent gel with immobilized phenylboronic acid groups.
    • Utilizing the gel's specific binding of cis-diols in ribonucleosides.
    • Quantitative recovery of bound nucleosides by elution with acetic acid.
    • Assaying nucleosides at pH 8.8 in a 0.25 mol/liter salt solution.

    Main Results:

    • The phenylboronic acid gel exhibits high capacity (0.26 mmol/ml) in its swollen state.
    • Nucleosides with a free diol group are retained, while free bases show minimal retention.
    • Pseudouridine is eluted at a unique position, allowing for its specific measurement.
    • Quantitative recovery of added nucleosides (neutral, cationic, anionic) was achieved.

    Conclusions:

    • The developed adsorbent gel provides a highly efficient and specific method for urinary nucleoside analysis.
    • This technique facilitates simple and rapid quantitation, potentially improving cancer detection.
    • The method is chemically mild and applicable to the measurement of specific nucleosides like pseudouridine.

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