Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Lectin histochemistry in the aged dog brain

W Kiatipattanasakul1, H Nakayama, S Nakamura

  • 1Department of Veterinary Pathology, Faculty of Agriculture, University of Tokyo, Japan.

Acta Neuropathologica
|May 30, 1998
PubMed
Summary

Lectins, which bind to sugars, reveal age-related changes in canine brain cells and amyloid plaques. These findings suggest lectins can serve as markers for studying canine brain aging and beta-amyloid deposition.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Primary structure of a gamma subunit of G protein, gamma 12, and its phosphorylation by protein kinase C.

The Journal of biological chemistry·1995
Same author

Induction of tissue inhibitor of metalloproteinase 3 gene expression during in vitro decidualization of human endometrial stromal cells.

Endocrinology·1995
Same author

[Histopathological studies on senile plaques and cerebral amyloid angiopathy in aged cynomolgus monkeys].

Experimental animals·1995
Same author

Aphasia in acute stroke: incidence, determinants, and recovery.

Annals of neurology·1995
Same author

Morphologic alterations and cytokinetic studies of tracheal autograft epithelium in rabbits.

The Annals of thoracic surgery·1995
Same author

Reduced expression of kan-1 (encoding putative bile acid-CoA-amino acid N-acyltransferase) mRNA in livers of rats after partial hepatectomy and during sepsis.

The Biochemical journal·1995

Area of Science:

  • Neuroscience
  • Histochemistry
  • Glycobiology

Background:

  • Brain aging involves changes in cellular composition and metabolism.
  • Amyloid plaques are a hallmark of neurodegenerative diseases, including in aged canines.
  • Lectins are proteins that bind to specific carbohydrate structures, offering potential as cellular markers.

Purpose of the Study:

  • To investigate age-dependent changes in canine brain cells using lectin histochemistry.
  • To identify lectins that can serve as markers for senile changes in the canine brain.
  • To explore the association between lectin binding and amyloid plaque deposition in aged dogs.

Main Methods:

  • Histochemical analysis of formalin-fixed, paraffin-embedded canine brains.
  • Application of 15 selected lectins to label cellular components.

Related Experiment Videos

  • Correlation analysis between lectin binding, glial/neuronal markers, and amyloid plaques.

    • Main Results:

    • Several lectins (Concanavalin A, Lycopersicon esculentum agglutinin, Limulus polyphemus agglutinin) showed age-dependent labeling of neurons and glial cells.
    • Amyloid plaques were positive for multiple lectins (Con A, Dolichos biflorus agglutinin, Glycine maximus agglutinin, LEL, PHA-L, Limax flavus agglutinin, Vicia villosa agglutinin).
    • Vicia villosa agglutinin, Glycine maximus agglutinin, and Limax flavus agglutinin intensely stained amyloid in blood vessels and plaque cores.

    Conclusions:

    • Monosaccharide composition and metabolism in canine brain cells change with age.
    • Specific lectins can be valuable histochemical markers for studying canine brain aging.
    • Certain lectins highlight the role of specific sugar residues in beta-amyloid deposition in aged dog brains.