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Updated: Feb 10, 2026

Capsular Serotyping of Streptococcus pneumoniae by Latex Agglutination
Published on: September 24, 2014
Long-term survival of Streptococcus pneumoniae at room temperature on Dorset egg medium
A D Wasas1, R E Huebner, M De Blanche
1MRC/SAIMR/WITS Pneumococcal Diseases Research Unit, South African Institute for Medical Research, Hillbrow, Johannesburg.
Abstract:
Forty-five isolates of Streptococcus pneumoniae were inoculated on Dorset egg and supplemented Columbia agar base media, incubated overnight at 37 degrees C, and then kept at room temperature (RT; 21 degrees C) or 4 degrees C. Long-term viability was best at RT for both media, with all isolates remaining viable on Dorset egg medium for 44 days; viability was 90 and 57% on Columbia agar base medium after 7 and 30 days. We recommend the use of Dorset egg medium for the maintenance of pneumococci at RT.
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