Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Behavior of supercoiled DNA

T R Strick1, J F Allemand, D Bensimon

  • 1Laboratoire de Physique Statistique de l'ENS, URA 1306 CNRS, Associé aux Universités Paris VI et VII, France. strick@clipper.ens.fr

Biophysical Journal
|April 17, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transcription-Coupled Repair: From Cells to Single Molecules and Back Again.

Journal of molecular biology·2019
Same author

Single-molecule characterization of extrinsic transcription termination by Sen1 helicase.

Nature communications·2019
Same author

Preparation of DNA Substrates and Functionalized Glass Surfaces for Correlative Nanomanipulation and Colocalization (NanoCOSM) of Single Molecules.

Methods in enzymology·2017
Same author

Stopped in its tracks: the RNA polymerase molecular motor as a robust sensor of DNA damage.

DNA repair·2014
Same author

Validation of a clinical evaluation score for irritative dermatitis: SCOREPI.

Journal of the European Academy of Dermatology and Venereology : JEADV·2012
Same author

Topological characterization of the DnaA-oriC complex using single-molecule nanomanipuation.

Nucleic acids research·2012

Researchers quantitatively studied DNA supercoiling using magnetic beads to control and measure torsional forces on single DNA molecules. This technique precisely quantifies piconewton forces, revealing insights into DNA mechanics under stretching.

Area of Science:

  • Molecular Biology
  • Biophysics
  • Nanotechnology

Background:

  • DNA supercoiling is crucial for DNA packaging and function.
  • Quantitative measurement of forces on single DNA molecules is challenging.
  • Understanding DNA mechanics under torsional stress is essential.

Purpose of the Study:

  • To quantitatively study DNA supercoiling using a novel micromanipulation technique.
  • To measure piconewton forces on single DNA molecules.
  • To investigate the effects of stretching forces on supercoiled DNA.

Main Methods:

  • Micromanipulation of single linear DNA molecules using magnetic field gradients.
  • Anchoring DNA to magnetic beads and a glass surface for torsional control.
  • Utilizing rotating magnetic fields for controlled over- and underwinding.

Related Experiment Videos

  • Measuring stretching forces via tethered-particle motion analysis.

    • Main Results:

    • Developed a powerful technique for precise piconewton force measurements.
    • Quantitatively controlled and monitored DNA supercoiling degree.
    • Studied the impact of stretching forces (0.01 pN to 100 pN) on supercoiled DNA.
    • Observed effects like stretching-relaxing hysteresis and DNA braiding.

    Conclusions:

    • The magnetic bead micromanipulation technique offers high precision for studying DNA mechanics.
    • Stretching forces significantly influence supercoiled DNA behavior.
    • The method provides a robust platform for exploring DNA structural dynamics.