Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

RB and apoptotic cell death

Q P Dou1, B An

  • 1Department of Pharmacology, University of Pittsburgh School of Medicine, and University of Pittsburgh Cancer Institute, Pittsburgh, PA 15213-2582, USA.

Frontiers in Bioscience : a Journal and Virtual Library
|April 18, 1998
PubMed
Summary

Cellular balance relies on coordinating proliferation and apoptosis. The Retinoblastoma protein (RB) regulates cell cycle and apoptosis, with its different forms influencing cell fate.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Influence of Blood Pressure and Heart Rate on PWV Measurement: Assessment Under Real-Time Blood Pressure Monitoring.

Physiological research·2025
Same author

[Smeglutide inhibits high glucose-induced proliferation of myocardial fibroblasts by downregulating TGFβ/Smad3 signaling].

Nan fang yi ke da xue xue bao = Journal of Southern Medical University·2023
Same author

[Epidural block associated with improved long-term survival after surgery for colorectal cancer: A retrospective cohort study with propensity score matching].

Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences·2021
Same author

Encapsulation of bacterial cells in cytoprotective ZIF-90 crystals as living composites.

Materials today. Bio·2021
Same author

On the mechanical behavior of austenitic stainless steel with nano/ultrafine grains and comparison with micrometer austenitic grains counterpart and their biological functions.

Journal of the mechanical behavior of biomedical materials·2019
Same author

Genome-wide association study reveals candidate genes associated with body measurement traits in Chinese Wagyu beef cattle.

Animal genetics·2019

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Cell number homeostasis depends on balancing cell proliferation and death.
  • Dysregulation of cell cycle progression is critical for initiating apoptosis.
  • Retinoblastoma protein (RB) is a key tumor suppressor and cell cycle regulator involved in apoptosis.

Purpose of the Study:

  • To investigate the regulatory role of Retinoblastoma protein (RB) in apoptosis.
  • To understand how RB's phosphorylation state and cleavage impact cellular fate during apoptosis.

Main Methods:

  • Analysis of RB protein modifications during apoptosis onset.
  • Investigation of RB cleavage by caspase family proteases.
  • Assessment of the anti-apoptotic function of unphosphorylated RB.

Main Results:

  • Hyperphosphorylated RB converts to a hypophosphorylated form during apoptosis, mediated by phosphatase activity.
  • Hypophosphorylated RB is cleaved by caspase-like proteases.
  • Unphosphorylated RB (p110/unphos) remains uncleaved and acts as an apoptosis inhibitor.

Conclusions:

  • RB's phosphorylation state and proteolytic cleavage are crucial for determining cellular fate during apoptosis.
  • Regulation of RB proteolytic activity and levels is vital for controlling apoptosis and maintaining cell number homeostasis.

Related Experiment Videos