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Legume lectin structure

R Loris1, T Hamelryck, J Bouckaert

  • 1Laboratorium voor Ultrastruktuur, Vlaams Interuniversitair Instituut voor Biotechnologie, Vrije Universiteit Brussel, Sint-Genesius-Rode, Belgium. reloris@vub.ac.be remy@ultr.vub.ac.be

Biochimica Et Biophysica Acta
|April 18, 1998
PubMed
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Legume lectins, carbohydrate-binding proteins, exhibit diverse specificities due to structural variations. Their quaternary structures influence interactions with carbohydrates and plant hormones, impacting cell activities.

Area of Science:

  • Biochemistry
  • Structural Biology
  • Molecular Biology

Background:

  • Legume lectins are homologous carbohydrate-binding proteins primarily found in legume seeds.
  • They display significant variations in carbohydrate specificity and quaternary structure despite conserved amino acid sequences and tertiary structures.

Purpose of the Study:

  • To review the structural features of legume lectins and their carbohydrate complexes.
  • To explore how structural variations contribute to diverse specificities and biological activities.

Main Methods:

  • Focus on structural analysis of legume lectins and their complexes.
  • Integration of recent mutagenesis data to elucidate structure-function relationships.

Main Results:

Related Experiment Videos

  • Monosaccharide specificity is determined by conserved hydrogen-bonding residues and a variable loop defining the binding site.
  • Higher affinity for specific oligosaccharides and hydrophobic aglycons arises from adjacent subsites.
  • Quaternary structure dictates specificity for multivalent carbohydrates and influences interactions with plant hormones.

Conclusions:

  • Structural plasticity in legume lectins underlies their diverse carbohydrate specificities.
  • Quaternary structure plays a critical role in biological activities, including mitogenic responses and hormone interactions.
  • The molecular basis for quaternary structure variation remains an area for further investigation.