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Nociceptin hyperpolarises neurones in the rat ventromedial hypothalamus
K Lee1, J R Nicholson, A T McKnight
1Parke Davis Neuroscience Research Centre, Cambridge University Forvie Site, UK.
Neuroscience Letters
|April 18, 1998
Abstract:
Patch-clamp recording techniques were used to examine the effect of nociceptin upon neurones contained in slices from the rat ventromedial hypothalamus (VMH). Bath application of 50-300 nM nociceptin hyperpolarised neurones in a concentration-dependent manner that was not affected by either tetrodotoxin (TTX) or naloxone. In voltage-clamp studies nociceptin induced an outward current at -60mV that had a reversal potential of -100.2 +/- 4.6 mV and was abolished by bath application of 2 mM BaCl2.