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[Analysis of digoxin by a radio-immunologic]
European Journal of Toxicology and Environmental Hygiene. Journal Europeen De Toxicologie
|May 1, 1976
Summary
Radiocompetition assays provide accurate insights into cardiotonic heteroside effects. This study compared commercial kits and analyzed cross-reactions to establish therapeutic and toxic levels in humans.
Area of Science:
- Pharmacology
- Biochemistry
- Clinical Chemistry
Background:
- Cardiotonic heterosides are crucial for treating cardiac conditions.
- Accurate quantification of these compounds is essential for therapeutic monitoring.
- Radioligand assays, specifically radiocompetition, offer a sensitive method for drug measurement.
Purpose of the Study:
- To compare the performance of two commercial radiocompetition kits for cardiotonic heterosides.
- To assess the specificity of these kits by evaluating cross-reactivity with endogenous and exogenous structural analogues.
- To determine the therapeutic and toxic concentration ranges of cardiotonic heterosides in human subjects.
Main Methods:
- Utilized radiocompetition assays to measure cardiotonic heterosides.
- Compared two distinct commercial assay kits based on established quality criteria.
- Investigated cross-reactivity profiles with structurally related compounds.
- Correlated assay results with clinical data to define therapeutic and toxic levels.
Main Results:
- Performance characteristics of the two commercial kits were evaluated.
- Significant cross-reactivity was observed with certain structural analogues, impacting assay specificity.
- Defined reference ranges for therapeutic and toxic levels of cardiotonic heterosides in patients.
Conclusions:
- Radiocompetition assays are valuable tools for assessing cardiotonic heterosides.
- Kit selection requires careful consideration of cross-reactivity for accurate clinical application.
- Establishing therapeutic and toxic levels aids in optimizing patient treatment and safety.