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Fluorescently detectable magnetic resonance imaging agents

M M Hüber1, A B Staubli, K Kustedjo

  • 1Division of Biology and Beckman Institute, California Institute of Technology, Pasadena 91125, USA.

Bioconjugate Chemistry
|April 21, 1998
PubMed
Summary
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Researchers developed novel bifunctional contrast agents for simultaneous optical and magnetic resonance imaging (MRI) in animal studies. These agents allow high-resolution imaging of biological structures at different depths, aiding developmental biology research.

Area of Science:

  • Biomedical imaging
  • Molecular imaging
  • Developmental biology

Background:

  • Simultaneous optical and magnetic resonance imaging (MRI) offers complementary resolution and depth information.
  • Bifunctional contrast agents are needed to integrate these imaging modalities effectively.
  • Understanding cellular dynamics during embryonic development requires advanced imaging tools.

Purpose of the Study:

  • To synthesize and characterize novel bifunctional contrast agents for combined optical and MRI.
  • To evaluate the utility of these agents for in vivo imaging of cellular processes.
  • To investigate cellular layer movements during amphibian gastrulation and neurulation.

Main Methods:

  • Synthesis of low-molecular weight (DOTA-tetramethylrhodamine) and high-molecular weight (poly(D-lysine) or dextran-based) bifunctional agents.

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  • Characterization of the synthesized contrast agents.
  • In vivo testing in Xenopus laevis embryos, including single-cell injection and imaging via optical and MRI microscopy.

    • Main Results:

    • Successful synthesis and characterization of bifunctional agents integrating gadolinium (Gd3+) chelators and fluorescent dyes.
    • Demonstrated simultaneous visualization of biological structures using both optical and MRI microscopy.
    • Enabled tracking of cellular layer movements during Xenopus laevis gastrulation and neurulation.

    Conclusions:

    • Bifunctional contrast agents provide a powerful strategy for multimodal imaging, offering insights into biological processes at different scales.
    • These agents facilitate the study of dynamic cellular rearrangements during embryonic development.
    • The developed agents are valuable tools for advancing in vivo imaging and developmental biology research.