Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hydroxyl radical activity in thermo-catalytically bleached root-filled teeth

S W Dahlstrom1, G S Heithersay, T E Bridges

  • 1Department of Dentistry, University of Adelaide, South Australia.

Endodontics & Dental Traumatology
|June 1, 1997
PubMed
Summary

Hydrogen peroxide bleaching of root-filled teeth can cause root resorption by generating hydroxyl radicals. These radicals, particularly when teeth are discolored by blood, degrade connective tissue, potentially leading to resorption.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

External tooth resorption associated with a peripheral odontogenic fibroma: review and case report.

Australian dental journal·2017
Same author

Life cycles of traumatized teeth: long-term observations from a cohort of dental trauma victims - series 2.

Australian dental journal·2016
Same author

Splinting of teeth following trauma: a review and a new splinting recommendation.

Australian dental journal·2016
Same author

Life cycles of traumatized teeth: long-term observations from a cohort of dental trauma victims.

Australian dental journal·2016
Same author

Management of tooth resorption.

Australian dental journal·2007
Same author

Evaluation of a combined thiourea and hydrogen peroxide regimen to bleach bloodstained teeth.

Australian dental journal·2007

Area of Science:

  • Endodontics
  • Dental Materials Science
  • Biochemistry

Background:

  • Intra-coronal bleaching of root-filled teeth is linked to invasive cervical root resorption.
  • Hydrogen peroxide diffusion into the periodontium is hypothesized to cause tissue destruction and resorption.
  • Hydrogen peroxide can generate reactive hydroxyl radicals, which degrade connective tissue components like collagen.

Purpose of the Study:

  • To investigate hydroxyl radical generation during the intra-coronal bleaching of blood-discolored, root-filled human teeth.
  • To explore the role of blood components and cleaning agents in this process.

Main Methods:

  • Forty extracted human premolars were root-filled and twenty were discolored with blood.
  • Teeth underwent thermo-catalytic bleaching with 30% hydrogen peroxide in sodium salicylate solution.

Related Experiment Videos

  • Hydroxyl radical generation was quantified using high-performance liquid chromatography with electrochemical detection (HPLC-ECD).

    • Main Results:

    • Hydroxyl radicals were detected in 25 out of 40 bleached teeth.
    • Significant association found between hydroxyl radical production and blood-induced tooth discoloration.
    • Higher hydroxyl radical yields were observed when EDTA was used for pulp chamber cleaning prior to bleaching.

    Conclusions:

    • Hydroxyl radicals are generated during the thermo-catalytic bleaching of root-filled teeth.
    • This generation, especially in discolored teeth, may be a key mechanism in post-bleaching periodontal tissue destruction and root resorption.