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Related Experiment Videos

Effects of selenium deficiency on diaphragmatic function after resistive loading

F H Andrade1, A Anzueto, W Napier

  • 1Department of Physiology, Baylor College of Medicine, Houston, Texas, USA.

Acta Physiologica Scandinavica
|April 29, 1998
PubMed
Summary

Selenium deficiency impairs diaphragm function during exercise by reducing glutathione peroxidase activity. This highlights the critical role of selenium in protecting respiratory muscles from oxidative stress.

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Area of Science:

  • Physiology
  • Nutritional Biochemistry
  • Respiratory Medicine

Background:

  • Diaphragmatic fatigue is linked to increased reactive oxygen species (ROS).
  • The glutathione redox system, including selenium-dependent glutathione peroxidase, is a key defense against ROS.
  • Selenium deficiency may compromise this defense, potentially increasing diaphragm susceptibility to oxidative stress.

Purpose of the Study:

  • To investigate the impact of selenium deficiency on diaphragmatic glutathione peroxidase activity.
  • To determine if selenium deficiency exacerbates diaphragm dysfunction under mild exertional stress.
  • To evaluate the protective role of glutathione peroxidase against exercise-induced oxidative stress in the diaphragm.

Main Methods:

  • Sprague-Dawley rats were fed selenium-deficient or control diets for 12 weeks.

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  • Animals were subjected to unloaded or inspiratory resistive loading conditions.
  • Diaphragmatic contractility, glutathione peroxidase activity, and glutathione content were measured.

    • Main Results:

    • Selenium deficiency significantly decreased diaphragmatic glutathione peroxidase activity.
    • Neither selenium deficiency nor resistive loading alone impaired diaphragmatic contractility.
    • Combined selenium deficiency and resistive loading significantly reduced diaphragmatic force and shifted the force/frequency curve.

    Conclusions:

    • Selenium deficiency reduces diaphragmatic glutathione peroxidase activity.
    • Selenium deficiency combined with resistive loading impairs diaphragm muscle function due to oxidative stress.
    • A functional glutathione peroxidase is essential for protecting the diaphragm against the effects of resistive loading.