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Related Experiment Videos

Kappa and lambda rearrangement occur simultaneously in transformed pre-B cells

D Liu1, J Jenab, N Rosenberg

  • 1Department of Pathology and Graduate Program in Immunology, Tufts University School of Medicine, Boston, MA 02111, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|April 29, 1998
PubMed
Summary

Murine B cells preferentially use kappa light chains due to recombinase machinery targeting both kappa and lambda loci simultaneously but favoring kappa rearrangement. This preference, alongside selection pressures, shapes B cell development.

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Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • Murine B-lineage cells express either kappa or lambda immunoglobulin light chains.
  • Most B cells express kappa protein, suggesting regulation at the gene rearrangement level.
  • This preference could stem from recombinase machinery bias or temporal differences in rearrangement initiation.

Purpose of the Study:

  • To investigate the kinetics of kappa and lambda light chain gene rearrangement in murine B cells.
  • To determine whether the recombinase machinery targets kappa and lambda loci simultaneously or sequentially.
  • To elucidate the mechanisms underlying the preferential usage of kappa light chains.

Main Methods:

  • Utilized pre-B cells transformed with temperature-sensitive Abelson virus mutants.

Related Experiment Videos

  • Shifted cells to a non-permissive temperature to induce kappa and lambda gene rearrangement.
  • Employed competitive PCR to analyze the kinetics of gene rearrangement at both loci.

    • Main Results:

    • Kappa and lambda loci were found to rearrange at approximately the same time.
    • Some clones rearranged only the lambda locus after short induction periods.
    • The frequency of lambda gene rearrangement was three- to sixfold lower than kappa gene rearrangement.

    Conclusions:

    • The recombinase machinery targets both kappa and lambda light chain loci concurrently.
    • There is a preferential targeting of the kappa locus by the recombinase machinery.
    • Both preferential targeting and selection pressures contribute to kappa gene usage in B cells.