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[Phosphorylation of 5'-O-phosphonylmethylthymidine and its incorporation into HeLa cells DNA]
M V Ias'ko1, G V Sidorov, R V Kondratov
1Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
Bioorganicheskaia Khimiia
|April 29, 1998
Abstract:
[3H]5'-O-Phosphonylmethylthymidine with a specific activity of 71 Ci/mmol was obtained by isotope exchange. Its incubation with a HeLa cell culture resulted in the formation of [3H]-labeled 5'-O-(beta-phosphoryl-alpha-phosphonylmethyl)thymidine, 5'-O-(beta,gamma-diphosphoryl-alpha-phosphonylmethyl)thymidine, and [3H]DNA. This proved the ability of 5'-O-phosphonylmethylthymidine to undergo phosphorylation and incorporation into the DNA of human cells.