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Related Experiment Videos

Basic fibroblast growth factor is a morphogenic modulator in kidney vessel development

S Kloth1, J Gerdes, C Wanke

  • 1Institute of Anatomy, University of Regensburg, Germany. Sabine.Kloth@VKL.Uni-Regensburg.de

Kidney International
|April 29, 1998
PubMed
Summary
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Basic fibroblast growth factor (bFGF) plays a morphogenic role in kidney development, not a mitogenic one. Its inhibitory effects on vessel formation can be counteracted by vascular endothelial growth factor (VEGF) and specific hormones.

Area of Science:

  • Developmental Biology
  • Nephrology
  • Vascular Biology

Background:

  • Kidney organogenesis requires synchronized development of renal vasculature, nephrons, and collecting ducts.
  • Hormones and mitogenic peptides like basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) are implicated in kidney development.
  • The specific function of bFGF in kidney organogenesis remains largely uncharacterized despite receptor expression in developing kidney tissues.

Purpose of the Study:

  • To investigate the role of bFGF in kidney development and vascularization.
  • To elucidate the precise function of bFGF during the differentiation processes in the developing kidney.

Main Methods:

  • Utilized an organotypic culture system with microsurgically prepared renal cortex explants from neonatal rabbits.

Related Experiment Videos

  • Employed immunohistological techniques to analyze tissue composition and cellular differentiation.
  • Cultured explants serum-free under continuous medium perfusion to assess growth factor effects.

    • Main Results:

    • Basic fibroblast growth factor (bFGF) alone inhibited detectable vessel formation in cultured kidney explants.
    • The inhibitory effect of bFGF was reversed by the addition of vascular endothelial growth factor (VEGF) or hormones like retinoic acid and aldosterone/vitamin D3.
    • Combinations of bFGF with VEGF or hormones promoted the development of small vessel-like structures.

    Conclusions:

    • Basic fibroblast growth factor (bFGF) exhibits a morphogenic rather than a mitogenic function in kidney vessel development.
    • bFGF's role is context-dependent, requiring interaction with other signaling molecules for proper vascular patterning.
    • This study reveals a novel regulatory mechanism in kidney vascular development involving bFGF.