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Double enzyme-catalyzed microreactors using capillary electrophoresis

D S Zhao1, F A Gomez

  • 1Department of Chemistry and Biochemistry, California State University, Los Angeles 90032-8202, USA.

Electrophoresis
|April 29, 1998
PubMed
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This study demonstrates a novel double enzyme-catalyzed microreactor using capillary electrophoresis (CE). The capillary effectively functions as a microreactor for enzyme-catalyzed reactions, enabling easy product quantitation.

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Chemical Engineering

Background:

  • Enzyme-catalyzed reactions are crucial in biochemical processes.
  • Microreactors offer controlled environments for chemical synthesis and analysis.
  • Capillary electrophoresis (CE) is a powerful separation technique.

Purpose of the Study:

  • To evaluate a double enzyme-catalyzed microreactor concept within a CE system.
  • To showcase the capillary as a versatile microreactor for sequential enzymatic conversions.
  • To demonstrate the quantitation of reaction products using CE.

Main Methods:

  • Utilizing capillary electrophoresis (CE) for separation and detection.
  • Injecting substrate and enzyme plugs sequentially into the capillary.

Related Experiment Videos

  • Employing two model enzyme systems: hexokinase/apyrase and lactate dehydrogenase/glucose-6-phosphate dehydrogenase.
  • Quantifying reaction extents and product ratios via CE.

    • Main Results:

    • Successfully demonstrated the double enzyme-catalyzed microreactor concept in CE.
    • Achieved sequential enzymatic conversions within the capillary.
    • Validated the ease of quantitation for reaction products.
    • Showcased the conversion of ATP to ADP and AMP, and NADH/NAD cycling.

    Conclusions:

    • The capillary can be effectively utilized as a double microreactor for sequential enzymatic reactions.
    • CE provides a facile method for quantitating products from these microreactor systems.
    • This approach offers a promising platform for biochemical analysis and synthesis.