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Ethyl acetate extraction procedure and isocratic high-performance liquid chromatographic assay for testosterone
M R Baltes1, J G Dubois, M Hanocq
1Université Libre de Bruxelles, Institut de Pharmacie, Laboratoire de Chimie Bioanalytique, Toxicologie et Chimie Physique Appliquée, Brussels, Belgium.
Abstract:
An isocratic reversed-phase high-performance liquid chromatography (HPLC) method was developed and validated for separation of testosterone and its main metabolites over the nominal range 20 to 40 microg/ml and 280 to 4600 ng/ml, respectively. Mobile phase composition (phosphate buffer-methanol-acetonitrile, 50:38.5:11.5) was optimised by studying the influence of numerous chromatographic parameters. The most critical one was the ratio CH3CN/CH3OH. Good recoveries (around 90% for all compounds) and an improved specificity were assessed by a double ethyl acetate extraction of biological samples. According to the performance criteria tested, the method could be applied to enzymatic inhibition and induction in vitro studies.