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MHC class II expression in double mutant mice lacking invariant chain and DM functions
G Kenty1, W D Martin, L Van Kaer
1Department of Molecular and Cellular Biology, The Biological Laboratories, Harvard University, Cambridge, MA 02138, USA.
Journal of Immunology (Baltimore, Md. : 1950)
|April 29, 1998
Summary
Mice lacking invariant (Ii) chain and DM show defective B cell maturation. These double mutants offer insights into class II antigen presentation and B cell development.
Area of Science:
- Immunology
- Molecular Biology
- Cell Biology
Background:
- Invariant (Ii) chain and DM are crucial for class II MHC molecule maturation and peptide loading.
- Deficiencies in Ii chain or DM lead to structural and functional abnormalities in class II molecules.
Purpose of the Study:
- To characterize the cellular and biochemical properties of mice lacking both Ii chain and DM.
- To investigate the impact of combined Ii chain and DM deficiency on class II antigen presentation and B cell development.
Main Methods:
- Analysis of mature Aalphab Abetab dimers using SDS-PAGE.
- Functional assays to assess peptide binding and T cell stimulation.
- Evaluation of B cell maturation and development in mutant mouse strains.
Main Results:
- Double-deficient mice exhibit floppy Aalphab Abetab dimers, independent of TAP.
- Increased peptide binding and presentation activities in Ii-DM- double-deficient cells compared to Ii chain mutants.
- More severe defects in B cell maturation and development observed in double mutants.
Conclusions:
- Combined Ii chain and DM deficiency results in distinct cellular and biochemical phenotypes.
- These double mutant mice are valuable tools for studying nonconventional class II antigen presentation in vivo.
- The findings highlight the critical roles of Ii chain and DM in both class II maturation and B cell development.