IgG in human cornea stained by various techniques

Four eyes were divided into portions which were prepared by various techniques for immunofluorescence microscopy. One portion was frozen and processed by cryostat cutting, a second was fixed in alcohol-acetic acid and embedded in paraffin, and a third was soaked in saline for 2 days to elute IgG and was then processed by the alcohol-acetic acid technique. All tissues received stain for IgG by either the direct (one-layer) or indirect (two-layer) technique. In all instances IgG was demonstrated in the same areas of the cornea. We conclude that the presence of IgG in human cornea is not dependent on the method used for fixation or processing of the tissue.