Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Microminiaturized immunoassays using atomic force microscopy and compositionally patterned antigen arrays

V W Jones1, J R Kenseth, M D Porter

  • 1Microanalytical Instrumentation Center, Ames Laboratory-USDOE, Iowa, USA.

Analytical Chemistry
|April 29, 1998
PubMed
Summary

This study presents a novel immunoassay method combining atomic force microscopy (AFM) with patterned antibody arrays. This approach enables sensitive detection of antibody-antigen interactions with an integrated reference plane.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Creation and evaluation of a gender diversity focused cultural competency training for phlebotomists: Study protocol for a randomized controlled trial.

Heliyon·2025
Same author

Pediatric donor heart acceptance practices in the United States: What is really being considered?

Pediatric transplantation·2023
Same author

Intrinsic thermalization of the honeycomb optical lattice.

Physical review. E·2023
Same author

An in vitro study of the effect of the optimal irrigation solution conditions during canine articular surgery.

Veterinary research communications·2019
Same author

Pilot feasibility randomized clinical trial of negative-pressure wound therapy versus usual care in patients with surgical wounds healing by secondary intention.

BJS open·2018
Same author

Homeless.

The Hospital·2018

Area of Science:

  • Biotechnology
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Immunoassays are crucial for detecting specific biomolecular interactions.
  • Atomic Force Microscopy (AFM) offers high-resolution topographic imaging.
  • Developing sensitive and specific immunoassays with internal references remains a challenge.

Purpose of the Study:

  • To develop a novel immunoassay platform integrating AFM with patterned antigen arrays.
  • To minimize nonspecific protein adsorption for enhanced signal detection.
  • To establish a method for label-free, high-resolution detection of antibody-antigen binding events.

Main Methods:

  • Fabrication of gold substrates with patterned arrays of immobilized rabbit IgG using a photolithographic coupling agent.

Related Experiment Videos

  • Creation of micrometer-sized grids using octadecanethiol (ODT) monolayers to prevent nonspecific adsorption.
  • Utilizing AFM for topographic imaging to detect height changes upon antibody-antigen complex formation.

    • Main Results:

    • Successfully created patterned arrays of immobilized rabbit IgG on gold substrates.
    • Demonstrated minimization of nonspecific protein adsorption using ODT grids and Tween 80.
    • Achieved label-free detection of goat anti-rabbit IgG binding to immobilized rabbit IgG via AFM topographic imaging.

    Conclusions:

    • The developed AFM-based immunoassay platform provides a sensitive and specific method for detecting antibody-antigen interactions.
    • The integrated reference plane simplifies data analysis and enhances detection accuracy.
    • This strategy offers advantages in sample preparation, reusability, and potential for miniaturization.