Sister chromatid exchanges in rotogravure printing plant workers
K D Hammer1, N Mayer, E H Pfeiffer
1Universität Hamburg, Institut für Hygiene, Germany.
Summary
Printing workers exposed to toluene showed a higher genotoxic risk, indicated by increased sister chromatid exchange (SCE) rates. Urinary hippuric acid (HA) levels confirmed significant toluene exposure in this occupational group.
Area of Science:
- Occupational Health
- Toxicology
- Genetics
Background:
- Ambient air contains toluene, a volatile organic compound.
- Printing workers face occupational exposure to toluene.
- Toluene exposure is a potential genotoxic risk factor.
Purpose of the Study:
- To assess the individual burden of inhaled toluene in printing workers.
- To investigate the link between toluene exposure and genotoxic phenomena.
- To evaluate sister chromatid exchange (SCE) rates in relation to toluene exposure.
Main Methods:
- Monitoring of individual toluene burden via urinary hippuric acid (HA) excretion post-shift.
- Comparison of HA excretion and SCE frequencies between 42 printing workers and 45 control blood donors.
- Determination of SCE frequencies in peripheral lymphocytes.
Main Results:
- Exposed workers had significantly higher median HA excretion (1.94 g/g creatinine) than controls (0.45 g/g creatinine).
- Exposed workers exhibited higher SCE rates (10.13 counts/lymphocyte) compared to controls (6.84 counts/lymphocyte).
- The observed differences in HA and SCE were statistically significant (P = 0.000), independent of smoking and alcohol consumption.
Conclusions:
- A strong relationship exists between individual toluene burden and genotoxic risk in printing workers.
- Toluene exposure, potentially from ink mist, contributes to genotoxic effects.
- Further investigation into ink mist as a source of genotoxicity is warranted.
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