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[Establishing the types of Dienes compatibility in clinical Proteus mirabilis strains]
1Zakład Mikrobiologii Lekarskiej AM w Lodzi.
Abstract:
The aim of the study was to use the Dienes phenomenon for the differentiation of Proteus mirabilis strains isolated from urine by the method of making possible their typing. The subject of the study were 351 strains of Proteus mirabilis obtained from the laboratory of the Institute of Pediatrics, Medical Academy in Lódź. For establishing of the compatibility type 18 indicator strains were taken, each of them had action antagonistic towards all other ones. The results were read according to the presence or absence of the demarcation line between indicator strains and the studied strain. Out of 351 strains in 190 (54%) the reactions were compatible with one or more indicator strains. They were classified into 30 Dienes compatibility types. In the view of the simplicity and easy performance the method can be used in every microbiological laboratory.
Insights
The Dienes phenomenon effectively differentiates and types 351 Proteus mirabilis urine isolates into 30 distinct compatibility types. This simple method is easily applicable in any microbiology lab.
Area of Science:
- Microbiology
- Bacteriology
Background:
- Proteus mirabilis is a significant cause of urinary tract infections.
- Accurate strain differentiation is crucial for epidemiological studies and infection control.
Purpose of the Study:
- To evaluate the Dienes phenomenon for typing Proteus mirabilis strains.
- To establish a method for differentiating Proteus mirabilis isolates from urine samples.
Main Methods:
- Utilized 351 Proteus mirabilis strains isolated from urine.
- Employed 18 indicator strains for Dienes phenomenon testing.
- Assessed compatibility based on the presence or absence of demarcation lines.
Main Results:
- Successfully classified 190 out of 351 (54%) strains.
- Established 30 distinct Dienes compatibility types.
- Demonstrated the method's efficacy in strain differentiation.
Conclusions:
- The Dienes phenomenon provides a simple and effective method for typing Proteus mirabilis.
- This technique is readily implementable in standard microbiological laboratories.
- Facilitates epidemiological tracking and management of Proteus mirabilis infections.