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Updated: Aug 26, 2026

Following Cell-fate in E. coli After Infection by Phage Lambda
Published on: October 14, 2011
Use of bacteriophage lambda recombination functions to promote gene replacement in Escherichia coli
1Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester 01655, USA. kenan.murphy@ummed.edu
Abstract:
Replacement of Escherichia coli's RecBCD function with phage lambda's Red function generates a strain whose chromosome recombines with short linear DNA fragments at a greatly elevated rate. The rate is at least 70-fold higher than that exhibited by a recBC sbcBC or recD strain. The value of the system is highlighted by gene replacement with a PCR-generated DNA fragment. The deltarecBCD::Plac-red kan replacement allele can be P1 transduced to other E. coli strains, making the hyper-Rec phenotype easily transferable.
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