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Updated: Aug 13, 2026

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations
Published on: November 3, 2010
Genetic variants of chicken erythrocyte histone H5
E Górnicka-Michalska1, J Pałyga, H Luboń
1Department of Genetics, Wyzsza Szkoła Pedagogiczna, Kielce, Poland.
Abstract:
Two allelic electromorphs a and b of chicken erythrocyte histone H5 have been detected in a sodium dodecyl sulfate polyacrylamide gel. In an acid-urea gel, however, each of the allelic variants was found to be accompanied by a slower migrating form. A comparison of alpha-chymotrypsin-digested products of H5.a and H5.b revealed that they differed in N-terminal domains. The H5 variants were distributed differently not only in various chicken races but also in distinct lines within a breed. Allele H5b was about 2.6-4.6 as abundant as its counterpart H5a in most chicken populations examined. These proportions were distorted in two Leghorn lines: the ratio of H5b to H5a was only 1.6 in line H22 and increased up to 32 in line G99.
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