Related Experiment Video
Updated: Aug 19, 2026

Identification of Kinase-substrate Pairs Using High Throughput Screening
Published on: August 29, 2015
Protein kinase B and rac are activated in parallel within a phosphatidylinositide 3OH-kinase-controlled signaling
H Welch1, A Eguinoa, L R Stephens
1Inositide Laboratory, Department of Signaling, the Babraham Institute, Cambridge CB2 4AT, United Kingdom.
Abstract:
The GTPase Rac and the protein kinase B (PKB) are downstream targets of phosphatidylinositide 3OH-kinase in platelet-derived growth factor-stimulated signaling pathways. We have generated PAE cell lines inducibly expressing mutants of Rac. Use of these cell lines suggests that Rac is involved in both platelet-derived growth factor-stimulated membrane ruffling and the activation of p70(S6K) but not in the activation of PKB. Furthermore, expression of constitutively active alleles of PKB in PAE cells suggests that PKB is able to regulate the activity of p70(S6K) but not the cytoskeletal changes underlying membrane ruffling. Thus, our results indicate that Rac and PKB are on separate pathways downstream of phosphatidylinositide 3OH-kinase in these cells but that both of these pathways are involved in the regulation of p70(S6K).
Related Concept Videos
Interactions Between Signaling Pathways
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Amplifying Signals via Second Messengers
Amplifying Signals via Enzymatic Cascade
MAPK Signaling Cascades
PI3K/mTOR/AKT Signaling Pathway
cAMP-dependent Protein Kinase Pathways

