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Identification and characterization of Arcobacter isolates from broilers by biochemical tests, antimicrobial
Summary
Biochemical profiling and antimicrobial resistance testing are key for tracking Arcobacter butzleri in broilers. Most isolates studied lacked plasmids, suggesting these methods are vital for epidemiological analysis.
Area of Science:
- Microbiology
- Food Safety
- Veterinary Science
Background:
- Arcobacter butzleri is an emerging foodborne pathogen.
- Poultry meat is a significant reservoir for A. butzleri.
- Understanding A. butzleri epidemiology is crucial for public health.
Purpose of the Study:
- To characterize Arcobacter butzleri isolates from broilers.
- To evaluate biochemical and antimicrobial resistance profiles.
- To assess the utility of these methods for epidemiological analysis.
Main Methods:
- API CAMPY system for biochemical profiling.
- Growth and tolerance tests.
- Antimicrobial resistance pattern determination.
- Plasmid DNA analysis.
Main Results:
- Biochemical profiling and antimicrobial resistance testing showed high discriminatory power.
- 21 out of 89 isolates harbored small plasmids (up to 5 kbp).
- 68 isolates were plasmid-free.
Conclusions:
- Biochemical profiling and antimicrobial resistance testing are valuable tools for A. butzleri epidemiological studies.
- Plasmid carriage is not universal among broiler-associated A. butzleri.
- These methods can aid in tracking pathogen transmission in poultry.