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Macrophages during infection with Trichinella spiralis in mice
K Karmańska1, M Houszka, A Widyma
1W. Stefański Institute of Parasitology, Polish Academy of Science, Wrocław.
Abstract:
Behaviour of macrophages in experimental mice trichinellosis was investigated using the immunoenzymatic technique with monoclonal antibodies CD11b/CD18 within the framework of avidin-biotin-DAB. The maximum and earliest mobilization of macrophages 7 day post infection (dpi) was observed in the lamina propria of the intestinal mucosa. The highest level of macrophages in the muscles tissue was noted on day 21 of infection, however as early as in 28 dpi, their maximum level was observed inside the larval capsules. They line, especially between 35-42 dpi internal capsule surface.
Insights
Macrophages rapidly mobilize to the intestine early in trichinellosis infection. Later, they accumulate in muscle tissue and encapsulate Trichinella larvae within 35-42 days post infection.
Area of Science:
- Immunology
- Parasitology
- Cell Biology
Background:
- Trichinellosis is a parasitic disease caused by Trichinella nematodes.
- Macrophages play a crucial role in the host immune response to parasitic infections.
- Understanding macrophage dynamics is key to developing effective treatments for trichinellosis.
Purpose of the Study:
- To investigate the spatiotemporal behavior of macrophages during experimental mice trichinellosis.
- To characterize macrophage infiltration and localization in different tissues throughout the infection course.
Main Methods:
- Utilized an immunoenzymatic technique employing monoclonal antibodies CD11b/CD18.
- Employed the avidin-biotin-peroxidase complex (ABC) method for detection.
- Analyzed macrophage distribution in intestinal mucosa and muscle tissue at various time points post infection.
Main Results:
- Earliest and maximal macrophage mobilization was observed in the intestinal lamina propria at 7 days post infection (dpi).
- Macrophages significantly accumulated in muscle tissue by 21 dpi.
- Maximal macrophage presence within larval capsules was noted by 28 dpi, lining the internal capsule surface between 35-42 dpi.
Conclusions:
- Macrophages are rapidly recruited to the site of intestinal infection in trichinellosis.
- A distinct temporal and spatial pattern of macrophage accumulation occurs in muscle tissue, correlating with larval encapsulation.
- These findings highlight the critical role of macrophages in the host response and parasite containment during trichinellosis.