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High temperature conjugation of proteins with carbohydrates
1Department of Tumor Immunology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław. borat@immuno.iitd.pan.wroc.pl
Glycoconjugate Journal
|April 29, 1998
Abstract:
A new procedure was used to conjugate lactose and dextran with BSA without using coupling or activating reagents. The method is simple, rapid and cheap. Reducing sugars covalently bind to proteins when lyophilized together and briefly heated to a high temperature.